It could be said that James Bond is a cultural icon meant to reinforce a number of ideas about what it means to be a man. He’s surrounded by trappings of traditional masculinity- the perfectly fitted suit, small handgun (because a real man doesn’t need a Rambo-style machine gun to do the job), straight razor shave, uncomplicated and unsweet cocktail. For succeeding at manliness, he’s always rewarded by saving the day and having unrestricted sexual access to women.
In this scene, Silva threatens not only James Bond, but all of the masculine ideal, with rape and attempts to make that masculine ideal into an object instead of a subject. This act instantly identifies Silva as the villain- as he’s not only threatening England, but manliness as a concept. But Bond refuses to let Silva make him into an object- he returns the serve by implying that it might not be his “first time,” and by doing so, that there’s nothing inherently lesser or unmasculine about having homosexual relations. After this scene, Silva stops using emasculation as a technique and starts to go after Bond’s true weakness- his relationship with M.